Journal: Cellular and Molecular Neurobiology
Article Title: Characterization of Perineuronal Nets in the Paraventricular Nucleus of the Hypothalamus and their Alteration in Neurogenic Hypertension
doi: 10.1007/s10571-025-01628-z
Figure Lengend Snippet: Developmental regulation of PNNs in the PVN. a WFA staining (magenta) shows no visible PNNs at P6, with progressive appearance at P14 and maturation by 1Mo. b-c Quantification of WFA+ area (Time, p < 0.0001; Sex, p = 0.1140; Interaction, p = 0.6546) and PNN intensity (Time, p < 0.0001; Sex, p = 0.0874; Interaction, p = 0.7332) across developmental timepoints (each data point = average of 3 mice, 7–12 sections per mouse). Two-way ANOVA with Tukey’s multiple comparisons. * p > 0.05 vs. P6 for each sex. PVN-F denotes females, and PVN-M denotes males. d Representative images of PNN-enwrapped neurons at P14, 3Mo, and 25Mo. e-f Quantification of WFA+ area (%) and fluorescence intensity in 3- vs. 25-month-old naïve male mice (3Mo n = 4; 25Mo n = 5). Data are shown as mean ± SEM; circles represent individual mice (average of 7–12 bilateral PVN sections/mouse). DAPI (blue) indicates nuclei and HuC/D (green) indicates neurons
Article Snippet: Sections were mounted using VECTASHIELD Hardset Antifade Mounting Media with DAPI (Vector Laboratories, H-1500-10).
Techniques: Staining, Fluorescence